Fig. 6.

(A) Heat map displays changes in gene after shMEIS1 in MPNST cells versus shNT. (B) Graph shows confirmation of reduced ID1 and CCNE2 mRNA in MPNST cells by qRT-PCR after exposure to respective shRNAs. (C) Survival p.i with shID1, shMEIS1, and shNT, analyzed using an Alamar blue fluorescence assay. (D) Immunoblots show ID1, cleaved PARP, p27^Kip and β Actin in UN, shNT and shID1 treated MPNST cells (p.i. day 4, 6). (E) Phase contrast images show the rescue of cell survival in MPNST cells co-infected with shMEIS1 and shp27, versus shMEIS1/shNT. (F) Bar graph shows significant rescue in survival of MPNST in the presence of shp27. (G) Immunoblots confirm the downregulation of p27^Kip expression in cells infected with shp27 concurrent with absence of cleaved PARP. (H) Schematic showing of how MEIS1 is driving survival of MPNST cells through downstream regulation of ID1 and p27^Kip.