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. Author manuscript; available in PMC: 2016 Aug 3.
Published in final edited form as: Nat Struct Mol Biol. 2013 Jul 14;20(8):1008–1014. doi: 10.1038/nsmb.2611

Figure 4. Multiple acidic residues in TAND2 affect yeast growth.

Figure 4

Growth phenotype of yeast strains carrying TAND mutations as indicated. The strains were serially diluted (10-fold), spotted onto YPD medium, and grown at the indicated temperatures for 3 days. Since both TAND1 and TAND2 regions affect TBP binding29, effects of site-specific TAND2 mutations on growth were assayed both in the presence (lanes 4–9) and absence (lanes 13–18, 22–23) of TAND1. For direct comparison, a joint experiment was performed with the previously studied F57A mutant29.