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. Author manuscript; available in PMC: 2017 May 28.
Published in final edited form as: J Mater Chem B. 2016 Mar 15;4(20):3562–3574. doi: 10.1039/c6tb00006a

figure 2.

figure 2

the effects of ecm analogs on hmsc chondrogenesis under free swelling culture conditions after 14 days of culture. gene expression (by qpcr) for a) collagen ii and b) collagen x. the symbols (diamond (donor 1), square (donor 2), circle (donor 3)) represent the mean for each donor (n=4 technical replicates). the line (▬) represents the mean for all donors (n=3) and p values are relative to peg-only hydrogel. normalized expression is defined as relative expression at day 14 normalized to relative expression at 24 hours after encapsulation. c) immunohistochemistry staining for collagen ii (green) (top row) and for collagen x (green) (bottom row). cell nuclei (blue) were counterstained with dapi. scale bar = 20μm.