Skip to main content
. Author manuscript; available in PMC: 2017 May 28.
Published in final edited form as: J Mater Chem B. 2016 Mar 15;4(20):3562–3574. doi: 10.1039/c6tb00006a

figure 3.

figure 3

the effects of dynamic compressive loading on hmsc chondrogenesis in a biomimetic peg-based hydrogel containing 0.1 mm rgd and 1 wt% chs after 21 days of culture. a) gene expression (by qpcr) for collagen ii. normalized expression is defined as relative expression at day 21 normalized to relative expression for pre-encapsulated hmscs. b) immunohistochemistry staining for collagen ii (green). cell nuclei (blue) were counterstained with dapi. scale bar = 20μm. c) semi-quantitative analysis of the fraction of cells staining positive for collagen ii. d) immunohistochemistry staining for aggrecan (red). cell nuclei (blue) were counterstained with dapi. scale bar = 20μm. d). in b and c, the symbols (diamond (donor 1), square (donor 2), circle (donor 3)) represent the mean for each donor (n=4 technical replicates). the line (▬) represents the mean for all donors (n=3) and p values are relative to peg-only hydrogel.