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. Author manuscript; available in PMC: 2017 May 28.
Published in final edited form as: J Mater Chem B. 2016 Mar 15;4(20):3562–3574. doi: 10.1039/c6tb00006a

figure 4.

figure 4

the effects of dynamic compressive loading on hmsc hypertrophy in a biomimetic peg-based hydrogel containing 0.1 mm rgd and 1 wt% chs after 21 days of culture. a) gene expression (by qpcr) for collagen x. normalized expression is defined as relative expression at day 21 normalized to relative expression for pre-encapsulated hmscs. b) immunohistochemistry staining for collagen x (green). cell nuclei (blue) were counterstained with dapi. scale bar = 20μm. c) semi-quantitative analysis of the fraction of cells staining positive for collagen x. d) immunohistochemisty staining for runx2 (green). cell nuclei (blue) were counterstained with dapi. scale bar = 20μm. e) the concentration of active mmp13 in culture media accumulated from days 17-21. in a, c and e, the symbols (diamond (donor 4), square (donor 5), and circle (donor 6)) represent the mean of each donor (n=4 technical repeats). the line (▬) represents the average of all donors (n=3).