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. Author manuscript; available in PMC: 2016 Aug 31.
Published in final edited form as: Cell Rep. 2016 Jul 21;16(5):1416–1430. doi: 10.1016/j.celrep.2016.06.087

Figure 2. Smn deficiency induces MN hyperexcitability non-cell autonomously.

Figure 2

(A) Representative images of intracellularly filled control (−Dox) and Smn-deficient (+Dox) MNs differentiated from RNAi ES cells and cultured under mixed conditions for 5 days. Upper panel: Alexa-555 fill in red; lower panel: merged with GFP in green. Scale bar=20µm. (B) Superimposed membrane responses (upper traces) following current injection (lower traces) in control and Smn-deficient MNs as in A. Scale bars=20mV, 40pA, 40ms. (C) Current/voltage relationships for the MNs shown in B (Input resistance: −Dox= 534MΩ, +Dox= 782MΩ) and averages for input resistance (Rin) and time constant (τ) of control (n=15) and Smn-deficient (n=14) MNs at 5DIV. (D) Representative images of intracellularly filled control (−Dox) and Smn-deficient (+Dox) MNs differentiated from RNAi ES cells and cultured for 5 days after FACS purification. Upper panel: Alexa-555 fill in red; lower panel: merged with GFP in green. Scale bar= 20µm. (E) Superimposed membrane responses (upper traces) following current injection (lower traces) of control and Smn-deficient FACS purified MNs as in D. Scale bars= 20mV, 40pA, 40ms. (F) Current/voltage relationships for the two FACS purified MNs shown in E (Input resistance: −Dox=582MΩ, +Dox=598MΩ) and average input resistance and time constant of FACS purified control (n=15) and Smn-deficient (n=15) MNs at 5DIV. See also Figure S2.