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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: J Neurovirol. 2016 Feb 3;22(5):555–563. doi: 10.1007/s13365-016-0423-x

Fig. 1. Construction of recombinant HSV-1 over-expressing TRβ1 (A8).

Fig. 1

A. The construction was based on the HSV-1 BAC and Cre-Lox P system described in Materials and Methods. The BAC was inserted between the HSV-1 UL3 and UL4 genes and the resulting virus retained same capacity of gene expression and replication as the wild-type virus.

B. Quantitative RTPCR to measure the TRβ1 mRNA level in Vero cells infected with A1 or A8. Mock is a no infection control. The TRβ1was normalized by cellular gene PPIA.