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. 2016 Aug 4;7:1162. doi: 10.3389/fpls.2016.01162

FIGURE 2.

FIGURE 2

Viral small RNAs (vsiRNAs) accumulate at high levels in CLCuD-inoculated cotton plants. (A) A summary of the bioinformatics pipeline employed for the systematic identification of vsiRNAs reads from small RNA libraries recovered from CK and CLCuD-inoculated systemic leaves in cotton plants. (B) Size distribution of small RNAs in libraries prepared from CK and CLCuD-inoculated cotton plants with two biological replicates. CK-1 and CK-2 indicate two biological replicates of small RNA libraries without CLCuD-inoculated cotton plants. T-1 and T-2 indicate two biological replicates of small RNA libraries after CLCuD-inoculated cotton plants at 20 dpi. (C) Histogram representation of total vsiRNAs reads with two biological replicates in each size class. T-1 and T-2 indicate two biological replicates of small RNA libraries after CLCuD-inoculated cotton plants at 20 dpi. (D) Size distribution of total small RNAs in the library from CLCuD-inoculated cotton plants. Cotton reads indicate the reads mapped to the upland cotton genome. Virus reads indicate the reads mapped to the CLCuD genome.