FIGURE 4.

The relative expression level of CLCuD vsiRNAs and predicted vsiRNA targets in CK and CLCuD-inoculated cotton plants. (A) The relative expression level of CLCuD vsiRNAs by stem-loop reverse transcription PCR in CK and CLCuD-inoculated cotton plants. Values were normalized using the cotton U6 RNA as an internal reference. Error bars represent standard errors of three biological replicates and the asterisk indicates significant difference based on the Student’s t-test (P < 0.05). (B) The relative expression level of vsiRNA targets using qRT-PCR analysis in CK and CLCuD-inoculated cotton plants. The cotton histone gene (AF024716) was used as an internal reference. Error bars represent standard errors of three biological replicates, and asterisks indicate significant differences based on the Student’s t-test (P < 0.05).