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. 2016 Aug 4;7:1162. doi: 10.3389/fpls.2016.01162

FIGURE 8.

FIGURE 8

The role of the vsiRNA target candidate during CLCuD infection with virus-induced gene silencing. (A) Relative expression levels of vsiR3114 target Contig28334 using qRT-PCR analysis in the VIGS-treated cotton plants 20 days after Agrobacterium infiltration with CLCrV vectors. The cotton histone gene (AF024716) was used as an internal reference. Error bars represented standard errors of threeto clari biological replicates, and asterisks indicate significant differences based on the Student’s t-test (P < 0.05). (B) CLCuD accumulation in the Contig28334 silenced plants was estimated from the total genomic DNA by qRT-PCR. Values were normalized using the cotton histone gene (AF024716) as an internal reference. Error bars represented standard errors of three biological replicates and asterisk indicates significant difference based on the Student’s t-test (P < 0.05). (C) Validation of vsiR3114-guided target cleavage by 5′-RACE analysis. The number of 5′-RACE clones corresponding to each site is indicated by arrows.