Table 1.

Timeline of experimental procedures.

Day	Event
1-10	60 male CD-1 mice arrive and housed in standard sized cages in groups of 3 while habituating to facility
11-28	Mice remain housed in same groups of 3 and undergo standard behavioral testing
11	Open-field testing on the 30 subject mice, chosen randomly from the group of 60
14	Novel-object testing on the same 30 subject mice
19	All 60 mice habituated to the social test arena for 10 minutes
20	Social interaction testing; each subject mouse is paired with a novel stimulus mouse
25	Social approach-avoidance testing; each subject mouse is paired with a novel stimulus mouse (different partner to the previous social test)
29	30 subject mice placed in the vivarium and social group observations and census counts begin
29-48	Two hours of behavioral observations occur each day and census counts occur each day at threee separate time points: 2 h prior to dark cycle onset, 1 h post dark cycle onset, and 3 h post dark cycle onset
48	At the conclusion of the 2 h of behavioral observations, mice are euthanized via cervical dislocation and brains are collected for gene expression analysis