Figure 2.

Stimulation with agonists of OR2AG1 and OR1D2 led to an intracellular Ca²⁺ increase in HASMCs. (A) Repetitive stimulation with amyl butyrate (300 μM, duration: 30 s) elicited a reproducible transient increase in intracellular Ca²⁺ measured with the ratiometric Ca²⁺ indicator FURA-2AM. (B) Amyl butyrate, an agonist for OR2AG1, activated HASMCs in a dose-dependent manner with an EC50 of 253.39 μM (N = 6). (C) The application of the OR1D2 agonist bourgeonal (100 μM, duration: 30 s) led to an increase in intracellular Ca²⁺, and repetitive stimulation exerted a reproducible effect. (D) Bourgeonal was able to activate HASMCs in a dose-dependent manner with an EC50 of 0.5043 μM (N = 3–5). (E,F) The application of the OR1D2 agonists lilial (300 μM, duration: 30 s; E) and 4-phenylbutyrate (4-PBA; 300 μM, duration: 30 s; F) led to an intracellular Ca²⁺ increase in ratiometric Ca²⁺ imaging experiments. Bars indicate the application duration. Error bars represent the ± SEM of three to four independent experiments.