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. 2016 Aug 4;7:1176. doi: 10.3389/fpls.2016.01176

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Copyright © 2016 Zhang, Hong, Huang, Liu, Tian, Dai, Cao, Huang, Li and Song.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Silencing efficiency and specificity for selected SlTPS, SlTPP and SlTRE1 genes in VIGS-infiltrated plants. (A) Silencing efficiency for each of the selected trehalose-related genes in corresponding VIGS-infiltrated plants. (B,C) Silencing specificity for 4 SlTPS genes and for SlTPP2. Ten-day-old tomato plants were infiltrated with agrobacteria carrying TRV-SlTPSs/SlTPPs/SlTRE1 or TRV-GUS constructs and leaf samples were collected at 4 weeks after agroinfiltration. Transcript levels for the selected trehalose-related genes were analyzed by qRT-PCR using a tomato SlActin gene as an internal control. Expression levels of the selected trehalose-related genes in TRV-SlTPSs/SlTPPs/SlTRE1-infiltrated plants were shown as percentages of the levels in TRV-GUS-infiltrated plants. Data presented are the means ± SD from three independent experiments and ^∗ above the columns indicate significant differences at p < 0.05 level between the TRV-SlTPSs/SlTPPs/SlTRE1-infiltrated and TRV-GUS-infiltrated plants.