FIGURE 1.

Expressional verification of the glycolate oxidase (GLO) overexpression lines. The plants were grown in Kimura B nutrient solution under normal natural conditions [temperature of 30–35/23–26°C (day/night), photosynthetically active radiation of 600–1500 μmol m^-2 s^-1 and photoperiod of 12 h day/12 h night]. The fully expanded leaf was detached at four-leaf stage for assay of transcripts (A,B) and activity (C). The OsActin gene was used as an internal control. The data are means ± SD of three biological replicates, and representative of three independent experiments. Different letters on the top of columns indicate significant difference at p < 0.05 according to Duncan’s multiple range test.