FIGURE 7.

H₂O₂ and salicylic acid (SA) accumulation in the GLO overexpression lines. Five-leaf plants were grown in a growth chamber under a normal condition [30°C (day/12 h)/25°C (night/12 h), 400 μmol m^-2 s^-1 light intensity, and 65% humidity] for 3 days, then the temperature and light intensity were increased to 40°C (day/12 h)/30°C (night/12 h) and 900 μmol m^-2 s^-1, respectively, for 24 h. H₂O₂ and SA were measured before and after the treatments. (A) H₂O₂-3′-diaminobenzidine (DAB) staining and the result is representative of three independent experiments; (B,C) SA was determined by HPLC chromatography. The other legends are the same as those in Figure 1.