Fig. 1.

a IC50 doses of buparlisib for P3 (left) and U87 (right) glioma cells. b Relative cell number of P3 (left) and U87 (right) glioma cells exposed to buparlisib for 72 h. c Quantification of BrdU positive P3 (left) and U87 (right) glioma cells treated with buparlisib for 72 h with doses as indicated, and subsequently pulsed with BrdU. d Quantification of Annexin V- and PI-positive P3 (left) and U87 (right) glioma cells treated with buparlisib for 72 h with doses as indicated, and subsequently incubated with PI and Annexin V Alexa Fluor 488 conjugate. Error bars represent SEM. Red bars indicate p values for linear trends. All experiments were performed three times. *p < 0.05, **p < 0.01, ***p < 0.001