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. 2016 Jul 11;96(8):918–931. doi: 10.1038/labinvest.2016.69

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Copyright © 2016 United States & Canadian Academy of Pathology

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Dynamics of granulocytes and antigen-uptaking cells during an acute airway inflammation. (a–c) Time series of a projection of four focal planes. Contacts between granulocytes (red and yellow circles) and antigen-uptaking cells (stained with DQ OVA, yellow and yellow arrows) could be observed (Supplementary Movie 12). (d–f) Time series of a projection of eight focal planes. Contacts between two or more granulocytes (Supplementary Movie 13). (g) Quantification of the duration of contacts between granulocytes and antigen-uptaking cells (DQ OVA⁺ cell–Gr) or between granulocytes (Gr–Gr), significance was tested with Mann–Whitney U-test. (h–k) Antigen-uptaking cells (stained with DQ OVA, yellow in (h–i) and red in (j and k)) were contacted by neutrophils (Gr-1-immunoreactive, red circle, yellow in (h and i) and not stained in (j and k)) and eosinophils (Gr-1-negative, white circles).

Dynamics of granulocytes and antigen-uptaking cells during an acute airway inflammation. (a–c) Time series of a projection of four focal planes. Contacts between granulocytes (red and yellow circles) and antigen-uptaking cells (stained with DQ OVA, yellow and yellow arrows) could be observed (Supplementary Movie 12). (d–f) Time series of a projection of eight focal planes. Contacts between two or more granulocytes (Supplementary Movie 13). (g) Quantification of the duration of contacts between granulocytes and antigen-uptaking cells (DQ OVA⁺ cell–Gr) or between granulocytes (Gr–Gr), significance was tested with Mann–Whitney U-test. (h–k) Antigen-uptaking cells (stained with DQ OVA, yellow in (h–i) and red in (j and k)) were contacted by neutrophils (Gr-1-immunoreactive, red circle, yellow in (h and i) and not stained in (j and k)) and eosinophils (Gr-1-negative, white circles).