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. 2016 Jul 4;5(7):e239. doi: 10.1038/oncsis.2016.46

Figure 2.

Figure 2

Silencing of Nm23H1 expression in the CE48T and the CETE2 cells increases cell migration and invasion accompanied by changes in junctional proteins. (a) Reduced expression of Nm23H1 in the CE48T and the CETE2 cells was achieved. (b) RT–PCR analysis revealed that the mRNA expression level of Nm23H2 in the CE48T-shNm23 cells was unaffected. (c) Representative photos of migration and invasion assays. Original magnification, × 100. The increased folds of migration and invasion were larger in the CE48T cells. The bar graphs presented the mean values obtained from three independent determinations (**P<0.01; *P<0.05). (d) Western blot analysis for Nm23H1, CLDN1, CLDN7, E-cadherin, N-cadherin and β-actin. (e) Nm23H1 (green), CLDN1 (red), CLDN7 (red) and E-cadherin (red) in the ESCC-shNm23 and the ESCC-shControl cells were detected by immunofluorescence staining. The cells were also counterstained with DAPI (blue) to localize the nucleus. Scale bar, 20 μm.