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. 2016 Jul 25;5(7):e244. doi: 10.1038/oncsis.2016.49

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Copyright © 2016 The Author(s)

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The role of TLR9 on colony formation and transformation. (a) HNSCC 136 cells stably transduced with PLVUT'-GFP and PLVUT'-TLR9 and were induced with doxycycline. After 3 days the cells were seeded (41, 123 or 370 cells per well) and 3 weeks after a crystal violet coloration was performed. (b) The number of colonies for the HNSCC 136 PLVUT'-GFP (white bars) and the HNSCC 136 PLVUT'-TLR9 (black bars) were counted and reported as noted in the chart. (c) PLVUT'-GFP (left panel) and PLVUT'-TLR9 (right panel) HNSCC 136 cells were either induced with doxycycline (lower panel) or left untreated (upper panel). Cells were included in agar and pictures were taken 11 days post inclusion. (d) The mean area of the colonies doxycycline+were counted with the software image from the pictures taken 11 and 28 days post inclusion. The mean area of colonies from cells that expressed GFP are in black and TLR9 are in white. Data are representative of three independent experiments performed in triplicate. The mean±s.e.m. are shown. **P<0.001 and P<0.01 based on an unpaired Student's t-test.