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. 2016 Jul 25;5(7):e244. doi: 10.1038/oncsis.2016.49

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Copyright © 2016 The Author(s)

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Transcriptome changes induced by TLR9 re-expression. (a) Schematic representation of the experiment. HNSCC 136 PLVUT'-GFP and HNSCC 136 PLVUT'-TLR9 were induced for 36 h with doxycycline. The mRNA was extracted and analyzed by microarray. (b) 'the true log2 of the quantile normalized expression signals in four samples belonging to two groups, GFP & TLR9 (No ratio and no scaling). In order to enhance data reading, color separation (blue=low and red=high) was based on the 50th theoretical percentile of the expression matrix range' (c) cDNA from HNSCC 136 PLVUT'-GFP and HNSCC 136 PLVUT'-TLR9 induced for 36 h was subjected to qPCR to determine the level of expression of IFI16, STAT1, IRF7, IL-29, TLR9 and PLS3. The ratio of expression TLR9 divided by GFP was calculated and plotted on the chart. Data have been disposed on the GEO: number being generated.