Table 4.
IC50 values of different antioxidant activities of ANM and its fractions
| IC50 (μg/ml) | |||||
|---|---|---|---|---|---|
| Sample | DPPH scavenging | Hydroxyl scavenging | Nitric oxide scavenging activity | β-carotene bleaching scavenging activity | Iron chelating assay |
| ANM | 32.0 ± 1.8d | 97.5 ± 2.9d | 153.6 ± 4.0c | 103.6 ± 4.2c | 130.5 ± 4.1c |
| ANH | 951.5 ± 4.3a | 932.5 ± 3.3a | 465.5 ± 2.8a | 858.9 ± 3.8a | 430.2 ± 3.9a |
| ANC | 633.8 ± 3.9b | 812.5 ± 2.9b | 414.9 ± 2.8b | 726.2 ± 4.7b | 347.9 ± 2.2b |
| ANE | 39.5 ± 2.1c,d | 102.7 ± 3.3d | 151.8 ± 3.1c | 41.4 ± 1.9f | 130.1 ± 0.8c |
| ANA | 43.7 ± 2.9c | 173.4 ± 2.0c | 84.6 ± 3.7d | 54.7 ± 4.4e | 76.8 ± 4.5d |
| Rutin | - | 83.3 ± 4.2e | - | - | - |
| Ascorbic acid | 11.9 ± 0.5e | - | 56.9 ± 2.5e | - | - |
| EDTA | - | - | - | - | 56.0 ± 1.7e |
| Catechin | - | - | - | 90.2 ± 2.3d | - |
Values are presented as mean ± SD (n = 3). Means with different superscript (a–f) letters in the rows are significantly (P < 0.01) different from each other
ANM A. nitida methanol extract, ANH A. nitida n-hexane fraction, ANC A. nitida chloroform fraction, ANE A. nitida ethyl acetate fraction, ANA A. nitida aqueous fraction