Fig. 4.

Changes of mRNA expression of SIRT1 in liver after 14 weeks.

Levels of SIRT1 against beta-actin mRNA expression are shown in the above histogram. C: normal control group, standard rat diet for 14 weeks and treated with 0.9% saline 10 mL/kg every day (n = 10); H: high-fat diet + STZ group, high-fat diet for 14 weeks and treated with 0.9% saline 10 mL/kg every day (n = 10); HS: high-fat diet + STZ with simvastatin treated group, high-fat diet for 14 weeks and treated with simvastatin 0.9 mg/10 mL/kg every day (n = 10); HDL: high-fat diet + STZ with low dose of DSKT treated group, high-fat diet for 14 weeks and treated with DSKT 3.75 g/10 mL/kg every day (n = 10); HDM: high-fat diet + STZ with medial dose of DSKT treated group, high-fat diet for 14 weeks and treated with DSKT 7.5 g/10 mL/kg every day (n = 10); HDH: high-fat diet + STZ with high dose of DSKT treated group, high-fat diet for 14 weeks and treated with DSKT 15 g/10 mL/kg every day (n = 10). *P < 0.05 vs. Group C, †P < 0.05 vs. Group H, ‡P < 0.05 vs. Group HS, §P < 0.05 vs. Group HDL, ||P < 0.05 vs. Group HDM. DSKT, daisaikoto; SIRT1, sirtuin 1, silent mating type information regulation 2 homolog 1; STZ, streptozotocin.