Table 1.
Genes and primers used in Q-PCR validation
| Gene | Accession No. | Primer name | Primer sequence (5′ to 3′) | Product length (bp) |
|---|---|---|---|---|
| Fbln5 | NM_011812.4 | Fbln5-F1 | TAGAGCTCAAGGCTAGAAG | 94 |
| Fbln5-R1 | ACTTAGCGTCTCTACTCTG | |||
| Car3 | NM_007606.3 | Car3-F1 | CAGCCCTGGTCAGCATCT | 145 |
| Car3-R1 | ATTGGCGAAGTCGGTAGG | |||
| Cyp2c69 | NM_001104525.1 | Cyp2c69-F1 | CTGAGAAAGGCACGAAGT | 232 |
| Cyp2c69-R1 | GAATGAGGTCCAACGATA | |||
| Atp6v0a4 | NM_080467.3 | Atp6v0a4-F1 | ATTCTCAGCCTCTTCAATCA | 195 |
| Atp6v0a4-R1 | CAGAAACATGCCGATAAAGT | |||
| Gbp2b | NM_010259.2 | Gbp2b-F | AAATGGCCTCAGAAATCCAC | 107 |
| Gbp2b-R1 | TTGGATAGCAGACAGGATGT | |||
| Maoa | NM_173740.3 | Maoa-F1 | AGATTTCTAAGCCTACCTGT | 136 |
| Maoa-R1 | GAGCCCTAATTTCATTCTGT | |||
| Serpina6 | NM_007618.3 | Serpina6-F1 | ACACCACCAAAGACACTCC | 193 |
| Serpina6-R1 | ATCATCAGGCTGCTCCAT | |||
| Ifng | NM_008337.4 | Ifng-F1 | CCTGAAAGAAAGCAGTGTCT | 85 |
| Ifng-R1 | TTTGTCATTCGGGTGTAGTC | |||
| Il1b | NM_008361.4 | Il1b-F1 | ACAGTGATGAGAATGACCTG | 332 |
| Il1b-R1 | GTAGTGCAGTTGTCTAATGG | |||
| Cat | NM_009804.2 | Cat-F1 | GCAGTGATTTCACATAGGAT | 131 |
| Cat-R1 | GAGAGCTGGTAATCTCTACT | |||
| β-actin | NM_007393.5 | β-actin-F1 | GCTTCTAGGCGGACTGTTAC | 100 |
| β-actin-R1 | CCATGCCAATGTTGTCTCTT | |||
Forward (F1) and reverse (R1) primers used for quantitative PCR