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. 2016 Aug 4;12:39. doi: 10.1186/s13007-016-0139-1

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Library preparation flowchart of MiddRAD protocol B and the original ddRAD protocol. a MiddRAD protocol B. b The original ddRAD library constructing flowchart, this procedure is adopted by Peterson et al. Protocol B contains nine steps while the original ddRAD protocol contains 12 steps. Only size-selected fragments are as templates for PCR amplification in protocol B