Table 4.
MiddRAD-seq data summary in rice and maize
| Genome information | Oryza sativa | Zea mays |
|---|---|---|
| Genome size (Mb) | 383 | 2300 |
| % of repeats in genome | 39.11 | 85.00 |
| GC content (%) | 43.56 | 46.83 |
| Expected information | ||
| Enzyme pairs | AvaII + MspI | AvaII + MspI |
| Expected RAD tag size range (bp) | 460–680 | 500–680 |
| Expected no. of RAD tags | 60,925 | 284,179 |
| Tags density per 100 kb | 15.92 | 12.36 |
| % of tags in CDS | 25.10 | 3.04 |
| Observed information | ||
| Raw reads | 14,732,449 | 7,414,009 |
| Clean reads | 14,146,516 | 7,337,556 |
| Observed no. of tags | 66,547 | 290,001 |
| Tag average depth | 212.58 | 25.30 |
| Tags per 100 kb | 17.38 | 12.61 |
| Simplification ratio (%) | 2.43 | 1.77 |
| % of tags in CDS | 31.49 | 1.15 |
| % of tags in repeats | 15.83 | 31.44 |