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. 2016 Jun 28;115(3):303–311. doi: 10.1038/bjc.2016.198

Figure 3.

Figure 3

Effect of the combination of CYC065 and Taselisib in vitro. (A) Uterine serous carcinoma-ARK-1 and USC-ARK-2 were treated with the half-maximal inhibitory concentration (IC50) of CYC065, the IC50 of Taselisib (GDC0032) or the combination of the IC50s of the two compounds for 72 h. Cell counting was then carried as described in Materials and Methods. The incubation of USC-ARK-1 and USC-ARK-2 cells with the combination of CYC065 and Taselisib was significantly more effective in inhibiting cells' growth than each of the single agents (P<0.05). (B) Representative western blot analysis depicting changes in the expression levels of proteins involved in the Her2/PI3K/AKT/mTOR pathway (i.e., Her2/neu, pHer2/neu, S6 and pS6) and in the CCNE1 pathway (Rb, pRb and CCNE1) following the treatment of USC-ARK-1 and USC-ARK-2 with CYC065 (CYC), Taselisib (GDC) or the combination (combo) for 6 h. The combination of CYC065 and Taselisib was highly effective in inhibiting pRb and pS6 in both cell lines. CTRL, control.