Figure 6. Ceramide analog enhances FasL-induced apoptosis in tumor cells in a Fas-dependent mechanism.

(A) Tumor cells were treated as shown in the absence or presence of ceramide analog IG7 (10 μM) for approximately 24 h. Both floating and adherent cells were collected and stained with PI and Annexin V. Cells were then analyzed by flow cytometry. (B) Cells as shown in A are quantified for apoptosis. Percent apoptotic cell death was calculated as (% Annexin V⁺PI⁺ cells of treated cells) − (% Annexin V⁺PI⁺ cells in the absence of FasL). Column: mean; Bar: SD.