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. 2016 Aug 4;6:30957. doi: 10.1038/srep30957

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Copyright © 2016, The Author(s)

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(a) SEA-induced senescence as indicated by SA-β-Gal activities. Dimethyl Sulphoxide (DMSO) and Etoposide (ETO) served as the negative and positive control for senescence, respectively. (b) Statistical analysis for the percentage of SA-β-Gal positive cells among LX-2 cells treated with or without SEA (10 μg/ml). (c) SEA inhibited LX-2 cell proliferation as determined by MTT. (d,e) Cell cycle distribution was measured by flow cytometry analysis at 2 days following SEA treatment. Statistical differences between groups are shown as follows: *p < 0.05; **p < 0.01.