Table 1. Specific Sets of Primers of Real-Time PCR.
Gene names | Primer Sequences |
---|---|
Pan-macrophage related | |
F4/80 | (F)GCATCATGGCATACCTGTTC (R)AGTCTGGGAATGGGAGCTAA |
M1-type related | |
Il-6 | (F)CCAAGAACGATAGTCAATTCCAGA (R)CATCAGTCCCAAGAAGGCAAC |
Cxcl-10 | (F)CCAAGTGCTGCCGTCATTTT (R)CTCAACACGTGGGCAGGATA |
Cxcl-11 | (F)GCTGCTCAAGGCTTCCTTATGT (R)ACTTTGTCGCAGCCGTTACT |
Tnf-α | (F)ACTGAACTTCGGGGTGATCG (R)TGGTTTGTGAGTGTGAGGGTC |
iNos | (F)CGGCAAACATGACTTCAGGC (R)GCACATCAAAGCGGCCATAG |
M2-type related | |
Ccl-2 | (F)TACAAGAGGATCACCAGCAGC (R)ATTCCTTCTTGGGGTCAGCAC |
Ccl-22 | (F)GCTGTGGCAATTCAGACCTC (R)TGACGGATGTAGTCCTGGCA |
Cd163 | (F)ATGCTTCCATCCAGTGCCTC (R)CACAAACCAAGAGTGCCGTG |
Cd206 | (F)GTTCACCTGGAGTGATGGTTCTC (R)AGGACATGCCAGGGTCACCTTT |
Arg-1 | (F)CAGCACTGAGGAAAGCTGGT (R)CAGACCGTGGGTTCTTCACA |
Housekeeping gene | |
β-actin | (F)CATCCGTAAAGACCTCTATGCCAAC (R)ATGGAGCCACCGATCCACA |
F, forward primer; R, reverse primer.