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. 2016 Aug 4;6:30927. doi: 10.1038/srep30927

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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PM1 cells were transduced with the empty vector (pLKO.1) or vectors leading to the expression of the indicated shRNAs. Total RNA was extracted and cDNA was synthesized for quantification of CIB1, CIB2 and GAPDH mRNA levels. The ratio of CIB1/GAPDH mRNA (A) and CIB2/GAPDH mRNA (B) are expressed relative to those of non-transduced (NT) PM1 cells. The surface expression of CCR5 (C) and CXCR4 (D) was measured by flow cytometry. The mean fluorescence intensity (MFI) values represent the mean ± SEM of 3 independent experiments and are expressed relative to values obtained for non-transduced cells. *P < 0.05, **P < 0.01 versus sh-SCRAM (One-way ANOVA).