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. 2016 Aug 4;6:31093. doi: 10.1038/srep31093

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Copyright © 2016, The Author(s)

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H1299 and PC-9 were transfected with the empty vector (pcDNA) or pcDNA-GAS5-AS1 at the indicated concentrations for 48 h. (A,B) Half of the cells were collected and subjected to total RNA extraction. qRT-PCR analyses were performed to detect the expression levels of GAS5-AS1. (C,D) The other half of the cells were subjected to western blot analyses with specific antibody. The expression levels of ZEB1, N-cadherin, and Vimentin were determined in H1299 cells, whereas ZEB1, N-cadherin, and SNAIL1 were analyzed in PC-9 cells.