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. 2016 Aug 4;6:30776. doi: 10.1038/srep30776

Figure 5. Effects of B[a]P on cell phenotype and cell cycle in F258 cells.

Figure 5

Cells were treated or not with B[a]P 50 nM. (A) Cell attachment and spreading were analyzed by monitoring impedance of the cell monolayer with xCELLigence technology. The inset histogram plots the slope of cell index measured in presence or absence of B[a]P. There were 5 independent experiments. (B) Impact of B[a]P on cell cycle progression was evaluated by flow cytometry following IP staining. There were 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001. DMSO vs B[a]P-treated cells. (C) Analysis of B[a]P-induced cell spreading by observation of ultra-thin sections (90 nm) of each culture by transmission electron microscopy. N = nucleus. Number of independent experiments = 2.