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. 2016 Aug 4;6:31041. doi: 10.1038/srep31041

Figure 5. Delayed addition of GroEL-AD to the fibril forming reactions of each client protein.

Figure 5

In each panel, colored arrowheads denote the instant at which excess GroEL-AD was added to each corresponding color-coded trace of the experiment. (a) α-Synuclein. GroEL-AD (3-fold molar excess) was added at 0 (black), 3 (blue), 8 (green) and 24 (magenta) hours after initiating the experiment. (b) Aβ42 peptide. GroEL-AD (20-fold molar excess) was added at 0 (black), 0.5 (blue), 1.5 (green) and 8 (magenta) hours after initiating the experiment. (c) GroES in 0.4 M Gdn-HCl. GroEL-AD (4-fold molar excess) was added at 0 (black), 6 (blue), 10 (green) and 24 (magenta) hours after initiating the experiment.