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. 2016 Jul 13;72(Pt 8):591–597. doi: 10.1107/S2053230X16010189

Table 1. Macromolecule-production information.

A TEV protease site was included in the forward primer and is underlined in the sequence. The cleavage site is denoted |.

Source organism Homo sapiens
DNA source Synthesized (GenScript)
Forward primer GACGACGACAAGATGGAGAATCTTTATTTTCAGGGCGATCAAGTAAAGAAGAGGAAAAAA
Reverse primer GAGGAGAAGCCCGGTTAGTTCTTTTCACCAATCACAATGG
Expression vector pET-46 Ek/LIC
Expression host E. coli BL21 Rosetta2
Construct sequence MAHHHHHHVDDDDKMENLYFQ|GDQVKKRKKKRKDYQPNYFLSIPITNKEIIKGIKILQNAIIQQDERLAKAMVSDGSFHITLLVMQLLNEDEVNIGIDALLELKPFIEELLQGKHLTLPFQGIGTFGNQVGFVKLAEGDHVNSLLEIAETANRTFQEKGILVGESRSFKPHLTFMKLSKSPWLRKNGVKKIDPDLYEKFISHRFGEEILYRIDLCSMLKKKQSNGYYHCESSIVIGEK