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. 2016 Jul 13;72(Pt 8):591–597. doi: 10.1107/S2053230X16010189

Table 2. Crystallization.

Method Sitting drop
Plate type 24-well Cryschem plate (Hampton Research)
Temperature (K) 291
Protein concentration (mg ml−1) 20
Buffer composition of protein solution 500 mM NaCl, 5 mM Tris–HCl pH 8.0, 1 mM DTT
Composition of reservoir solution 2.5 M sodium malonate pH 7.0
Volume and ratio of drop 1:1, 2 µl total
Volume of reservoir (µl) 250