| Method |
Vapour diffusion |
| Plate type |
96-well MRC 2 Drop plate (screening), 24-well VDXm plate (production) |
| Temperature (K) |
293 |
| Protein concentration (mg ml−1) |
2.9 (BD1), 4.3 (NTPR) (1:3 ratio; 171 µM BD1 and 513 µM NTPR) |
| Buffer composition of protein solution |
25 mM HEPES pH 8.5, 150 mM NaCl, 0.5 mM TCEP, 10% glycerol |
| Composition of reservoir solution |
0.1 mM HEPES pH 7, 1.56 M ammonium sulfate |
| Volume and ratio of drop |
1:1 ratio protein:reservoir; 0.2 µl (screening), 2 µl (production) |
| Volume of reservoir |
70 µl (screening), 300 µl (production) |
