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. 2016 Jul 1;48(7):e243. doi: 10.1038/emm.2016.38

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Copyright © 2016 KSBMB.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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The effect of 3-MA treatment on LC3 expression, AHR and airway inflammation. (A) LC3 expression after 3-MA treatment. (B) Changes in lung resistance in response to increasing doses of methacholine were assessed 48 h after the final challenge. (C) Airway inflammatory cell counts in BALF. (D) Cytokine levels in BALF. (E) H&E-stained lung histology. (F) PAS-stained lung histology. (a) PBS/2% OVA-challenged mice. (b) OVA/1% OVA-challenged mice. (c) OVA/2% OVA-challenged mice. (d) Quantification of inflammatory cells or PAS-positive cells. The data are expressed as the mean±s.e.m. ^##P<0.01 versus the PBS/2% OVA, *P<0.05 versus the OVA/2% OVA. BALF, bronchoalveolar lavage fluid; H&E, hematoxylin and eosin; OVA, ovalbumin; PAS, periodic acid-Schiff; PBS, phosphate-buffered saline; 3-MA, 3-Methyladenine.