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. 2016 Jul 29;48(7):e248. doi: 10.1038/emm.2016.57

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Copyright © 2016 KSBMB.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Effects of miR-30c on the differentiation of P19 cells. (a) miR-30c overexpressing or knockdown P19 cells were stimulated to differentiate by DMSO for 10 days. Images are shown from days 0, 2, 4, 6, 8 and 10. Scale bar, 50 μm. (b) qRT-PCR analysis of expression levels of myocardial cell markers at the indicated time points during the induction of differentiation. The data are presented as the mean±s.d. of three experiments (*P<0.05 comparing miR-30c overexpression with control cells; ^#P<0.05 comparing miR-30c knockdown with control cells). (c) Immunostaining of myocardial cell markers cTnT and Gata4 at the indicated time points. DAPI was used to stain the nuclei. Scale bar, 50 μm.