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. 2016 Jul 29;48(7):e248. doi: 10.1038/emm.2016.57

Figure 5.

Figure 5

Effects of miR-30c on the Shh signaling pathway. (a, b) Identification of Gli2 as a potential target gene of miR-30c. (a) Dual luciferase reporter assay of miR-30c with a Gli2 3′ UTR reporter. The data are presented as the mean±s.d. of three experiments (*P<0.05 comparing miR-30c overexpression with control cells; #P<0.05 comparing miR-30c knockdown with control cells). (b) Western blotting of Gli2 protein levels in stable miR-30c overexpression or knockdown cell lines. (c) Immunostaining of Gli2 in stable miR-30c overexpression or knockdown cell lines. DAPI was used to stain the nuclei. Scale bar, 20 μm. (d, e) Ptch1 protein (d) and mRNA (e) levels were determined by western blotting or qRT-PCR, respectively, in miR-30c overexpression or knockdown cell line after treatment with DMSO for the indicated days. (*P<0.05 comparing Ptch1 mRNA levels in the cells on day 4 with those on day 0; #P<0.05 comparing Ptch1 mRNA levels in the cells on day 10 with those of day 0).