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. 2016 Jul 22;26(8):886–900. doi: 10.1038/cr.2016.87

Figure 5.

Figure 5

Activation of ADAM metalloproteases accelerates necroptotic cell death and promotes inflammation. (A) HT29 cells expressing shRNAs targeting ADAM9, ADAM10 or ADAM9 and ADAM10 were treated with TSZ at different time points and cell death was determined by PI staining. Results shown are averages ± SEM from three independent experiments. (B) Time-lapse microscopic analysis of cell morphologic changes after induction of necroptosis by TSZ in shRNA-control or shRNA -ADAM9/10 HT29 cells. (C) Representative dot plots of Ly-6G and 7/4 expression on peritoneal cavity cells and (D) neutrophil influx numbers in peritoneal cavity in C57BL/6 mice injected with PBS, necroptotic CRISPR-control- or CRISPR-ADAM10/17 MEF cells. (E) Mice were pre-treated with DMSO vehicle or GW280264X and followed by induction of acute pancreatitis by caerulein. Representative H&E-stained pancreatic sections from PBS or caerulein-treated mice were shown. (F) Serum amylase activity was measured in each group of mice (n = 5 per group).