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. 2016 Jul 28;7(7):e2317. doi: 10.1038/cddis.2016.205

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Copyright © 2016 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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CSCs are activated by c-Kit^TgD814Y. (a) Flow cytometry of CSCs isolated from c-Kit^+/+ and c-Kit^TgD814Y hearts. (b and c) Cell growth curve and BrdU incorporation evaluation of c-Kit^+/+ and c-Kit^TgD814Y CSCs. Data are reported±S.D. *P<0.05. (d and e) Clone and sphere formation assay was performed on c-Kit^+/+ and c-Kit^TgD814Y CSCs and the results are summarized in the histogram bar. Representative images of clones is shown. Data are reported±S.D. *P<0.05. (f) WB and densitometric analysis on protein extracts from c-Kit^TgD814Y and c-Kit^+/+ CSCs of three different experiments. (g and h) Clone and sphere formation assay was performed on c-Kit^TgD814Y CSCs after 14 days of treatment with AKT (MK2206, 1 μM), ERK inhibitor (PD98059, 100 μM) or the combination of them. Data are reported±S.D. *P<0.05 versus untreated sample (NT)