Figure 4.

Ectopic expression of FHL2 promotes GCT tumorigenesis. (a) Representative images showing tumor formation in COV434 cells transfected with an empty control vector (control). (b) Representative images showing tumor formation in COV434 cells transfected with an FHL2-expressing vectors (FHL2). The formed tumors from control and FHL2 groups were isolated and presented under the image of each group. The minimum scale of the rule is mm. (c) Growth curve of tumor xenografts derived from control and FHL2-expressing COV434 cells. Each point represents mean diameter±S.E.M. of six animals. ***P<0.001 compared with control (Ctrl) on day 29. (d) Weights of tumor xenografts derived from control and FHL2-expressing COV434 cells. Each bar represents mean±S.E.M. of six animals. **P=0.0116 compared with control (Ctrl). (e) Expression of Ki67 (red) in the tumor tissues derived from control and FHL2-expressing COV434 cells. Ki67 expression was determine using fluorescent immunohistochemistry. Nuclei were stained with DAPI (blue) and actin was stained with FITC-phalloidin (green). Scale bar: 20 μm