Figure 5.

Silencing of transferrin expression by siRNA decreases cell death induced by siramesine and lapatinib. MDA MB 231 cells were knockdown in (a) transferrin receptor (TfR) and (b) transferrin expression by siRNAs as demonstrated by western blot. (c) Knocked down of transferrin (siTransferrin) was performed. Cells were treated with DMSO (d), siramesine(S), lapatinib (L) and siramesine and lapatinib (S+L). The amount of cell death after 4 h was determined by trypan blue exclusion assays. Cells transfected with siControl were used as a control. (d) Knocked down of transferrin (siTransferrin) in SKBr3 cells was performed and the cells were treated as described above. The amount of cell death was determined at 4 h. Standard error represents three independent experiments (n=3). * represents statistical significance of P<0.05