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. 2016 Jul 28;7(7):e2311. doi: 10.1038/cddis.2016.219

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Copyright © 2016 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Ras-Cys118 is necessary for PGA₁-induced apoptosis. H-Ras^−/−/N-Ras^−/−MEFs transiently transfected with pCEFL-KZ-AU5 (Vector), pCEFL-KZ-AU5-H-Ras-wt (WT), pCEFL-KZ-AU5-H-Ras-C118S (C118S), or pCEFL-KZ-AU5-H-Ras-C184S (C184S) were treated as in Figure 2b. (a) Cell lysates were as in Figure 2b. Levels of cleaved caspase-3 are provided at the bottom (S.D. <10% average in each case, n=4). (b) Annexin V/FITC staining analysis was performed as in Figure 2c (mean±S.D., n=4). ***P≤0.001 versus non-stimulated (DMSO) cells