Skip to main content
. 2016 Jul 21;7(7):e2305. doi: 10.1038/cddis.2016.220

Figure 2.

Figure 2

TWEAK/Fn14 promotes h-VSMCs osteogenic transition. The effects of TWEAK (100 ng/ml, 7 days) were assessed both in non-calcific (Ct: 1.1 mmol/l Pi) and pro-calcific (Pi: 3.0 mmol/l Pi) conditions. (a–c) Effects of TWEAK on acta2 (a), myh11 (b) and bmp2 (c) mRNA expression, assessed by quantitative reverse transcriptase-PCR. *P<0.05, **P<0.01, ***P<0.001 vs cells exposed to 1.1 mmol/l Pi without TWEAK. $P<0.05, $$P<0.01, $$$P<0.001 vs cells exposed to 3.0 mmol/l Pi without TWEAK. †P<0.05, †††P<0.001 vs cells exposed to 1.1 mmol/l Pi with TWEAK. Results represent at least three independent experiments performed in triplicate. Error bars represent the S.E.M. (d) Effects of TWEAK on TNAP activity assessed by PNPP phosphatase assay. *P<0.05, **P<0.01 vs cells exposed to 1.1 mmol/l Pi without TWEAK. $P<0.05 vs cells exposed to 3.0 mmol/l Pi without TWEAK. †P<0.05 vs cells exposed to 1.1 mmol/l Pi with TWEAK. Results represent five independent experiments performed in triplicate. Error bars represent the S.E.M.