Figure 3.

TWEAK/Fn14 promotes MMP9 but not MMP2 activity. (a and b) Effects of TWEAK on mmp2 (a) and mmp9 (b) mRNA expression assessed by quantitative reverse transcriptase-PCR. **P<0.01 vs cells exposed to 1.1 mM Pi without TWEAK. $$$P<0.001 vs cells exposed to 3.0 mmol/l Pi without TWEAK. Results represent at least three independent experiments performed in triplicate. Error bars represent the S.E.M. (c) Effects of TWEAK on MMP2 and MMP9 activity in h-VSMCs supernatants, assessed by gel zymography. **P<0.01, ***P<0.001 vs cells exposed to 1.1 mM Pi without TWEAK. ^$$$P<0.001 vs cells exposed to 3.0 mmol/l Pi without TWEAK. ^††P<0.01 vs cells exposed to 1.1 mmol/l Pi with TWEAK. Results represent at least three independent experiments performed in triplicate. Error bars represent the S.E.M. NS, not significant