Figure 5.

TWEAK/Fn14-induced canonical NFκB activation favors TNAP and MMP9 activity. h-VSMCss were transfected with either scrambled or RelA siRNA and exposed or not for 7 days to TWEAK in pro-calcific conditions (3.0 mmol/l Pi). (a) Impact of RelA downregulation on TWEAK-modulation of TNAP activity. TNAP activity was assessed by pNPP phosphatase assay. *P<0.05 vs scrambled transfected cells without TWEAK. ^†P<0.05 vs scrambled transfected cells exposed to TWEAK. Error bars represent the S.E.M. (b) Impact of RelA downregulation on TWEAK-induced mmp9 mRNA expression assessed by quantitative reverse transcriptase-PCR. *P<0.05 vs scrambled transfected cells without TWEAK. ^†P<0.05 vs scrambled transfected cells exposed to TWEAK. Results represent three independent experiments performed in triplicate. Error bars represent the S.E.M. (c) Impact of RelA downregulation on TWEAK-induced MMP9 activity, assessed by gel zymography on cell supernatant. *P<0.05 vs scrambled transfected cells without TWEAK. ^†P<0.05 vs scrambled transfected cells exposed to TWEAK. Results represent three independent experiments performed in triplicate. Error bars represent the S.E.M. NS, not significant