Figure 7.

TWEAK/Fn14-induced non-canonical NFκB activation does not influence TNAP activity but favors mmp9 activity. h-VSMCss were transfected with either scrambled or RelB siRNA and exposed or not for 7 days to TWEAK in pro-calcific conditions (3.0 mmol/l Pi). (a) Impact of RelB downregulation on TWEAK-induced modulation of TNAP activity. TNAP activity was assessed by pNPP phosphatase assay. **P<0.01 vs scrambled transfected cells without TWEAK. ^$$P<0.05 vs cells transfected with siRNA RelB and not exposed to TWEAK. Error bars represent the S.E.M. (b) Impact of RelB downregulation on TWEAK-induced mmp9 mRNA expression assessed by quantitative reverse transcriptase-PCR. *P<0.05 vs scrambled transfected cells without TWEAK. ^$P<0.05 vs siRNA RelB transfected cells without TWEAK. ^†P<0.05 vs scrambled transfected cells exposed to TWEAK. Results represent three independent experiments performed in triplicate. Error bars represent the S.E.M. (c) Impact of RelB downregulation on TWEAK-induced MMP9 activity assessed by gel zymography in h-VSMCs supernatants. *P<0.05 vs scrambled transfected cells without TWEAK. ^†P<0.05 vs scrambled transfected cells exposed to TWEAK. Results represent three independent experiments performed in triplicate. Error bars represent the S.E.M. NS, not significant