Figure 2.
Sjp40 induces a senescence-like phenotype and inhibits cell growth in activated HSCs. (a) Effects of Sjp40 (20 μg/ml) on cell senescence in LX-2 cells were determined via senescence-associated β-galactosidase assay (original magnification × 200). Bar: 50 μm. (b) The graph represents quantitative analysis of the percentage of SA-β-Gal-positive cells (%). Data were expressed as the mean±S.E.M. of three or four independent trials. *P<0.05 compared with the control group. (c) Effect of Sjp40 (20 μg/ml) on the proliferation of LX-2 cells. LX-2 cells proliferation was examined using the MTT assay. Data were expressed as the mean±S.E.M. of three or four independent trials. **P<0.01 compared with the control group. (d) Flow cytometric analysis was applied to analyze Sjp40-induced cell cycle arrest of LX-2 cells in the G1 phase. (e) Quantitative analysis of cell cycle distribution graph describing the role of Sjp40 in regulating the G1 phase of cell cycle arrest. Data were expressed as the mean±S.E.M. of three or four independent trials. (f) The expression of Cyclin A and D1 by analysis of western blot. All values were expressed as the mean±S.E.M. of three or four independent trials. *P<0.05 compared with the control group