Figure 3.

Sjp40-induced senescence is dependent on the p53 and p21 pathway. (a) Western blot analysis of the expression of p53, P-p53, p21 and p16 in LX-2 cells treated with Sjp40. Data were expressed as the mean±S.E.M. of three or four independent trials. *P<0.05 compared with the control group. ^#P>0.05 compared with the control group. (b) Knockdown of p53 rescued the Sjp40-induced senescence analyzed by SA-β-Gal assay, and quantitative analysis of the percentage of SA-β-Gal-positive cells (%) was expressed as the mean±S.E.M. of three or four independent trials. *P<0.05 compared with ShRNA-p53 (Sh-p53). ***P<0.001 compared with ShRNA-Control (Sh-Con). Bar: 50  μm. (c) LX-2 cells were transfected with Sh-p53 or Sh-Con and additionally treated with or without Sjp40 for 48 h. The protein expression was investigated by western blot assay and data were expressed as the mean±S.E.M. of three or four independent trials. *P<0.05 compared with the control group; ^&P<0.05 compared with the Sh-con group. ^$P<0.05 compared with the control group. ^#P>0.05 compared with the Sh-p53 group. ^ϕP<0.05 compared with the Sjp40 group